Novel fructopyranose oligosaccharides isolated from fermented beverage of plant extract

Four oligosaccharides containing a fructopyranosyl residue have been found from fermented beverage of plant extract and isolated from the beverage using carbon-Celite column chromatography and preparative high performance liquid chromatography. Structure confirmation of the saccharides was provided by methylation analysis, MALDI-TOF-MS and NMR measurements. These saccharides were identified as oligosaccharides of fructopyranoside series; beta-D-fructopyranosyl-(2 -> 6)-D-fructofuranose (1), beta-D-fructopyranosyl-(2 -> 1)-D-fructopyranose (2), beta-D-fructopyranosyl-(2 -> 1)-beta-D-fructofuranosyl-(2 <-> 1)-alpha-D-glucopyranoside (3), and beta-D-fructopyranosyl-(2 -> 6)-alpha-D-glucopyranosyl-(1 <-> 2)-beta-D-fructofuranoside (4). Saccharides 3 and 4 among novel saccharides 1, 3, and 4 were named 'pyrano-1-kestose (pyrano-isokestose)' and 'pyrano-neokestose', respectively. (C) 2009 Elsevier Ltd. All rights reserved.