Journal
CARBOHYDRATE RESEARCH
Volume 345, Issue 2, Pages 275-283Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2009.10.009
Keywords
Agaro-oligosaccharides MALDI MS; Agaro-oligosaccharide alditols MALDI MS; Kappa-carrageenan oligosaccharides MALDI MS; Iota-carrageenan oligosaccharide alditols; MALDI MS; Nor-harmane; DHB
Funding
- Japan Society for the Promotion of Science (JSPS) [(S)-20228004]
- National Research Council of Argentina [CONICET
- PIP 5443]
- Agencia Nacional de Promocion Cientifica y Tecnologica [ANPCYT
- PICT06-0615]
- University of Buenos Aires [UBA X072, X137]
- Fundacao Araucaria/CNPq-Brasil
- MCT/CNPq/MS-SCTIE-DECIT/Cf-Saude-Brasil [554671/2006-9]
- CNPq-Brasil
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MALDI-TOF mass spectrometry analyses of several oligosaccharides (aldoses) and oligosaccharide alditols derived from agaroses, kappa- and iota-carrageenans using different matrices (2,5-dihydroxybenzoic acid, nor-harmane, ferulic acid, and the ionic liquid matrices 2,5-dihydroxybenzoic acid-n-butylamine and ferulic acid-n-butylamine) were conducted. These carbohydrates were selected as model compounds to study the MALDI prompt and post-source decay (PSD) fragmentation processes of both families of oligosaccharides. Sulfated alditols showed in the negative-ion mode the molecular ion as [M-Na](-) together with the species yielded by their prompt fragmentation (mainly desulfation) while the sulfated oligosaccharides (aldoses) showed mainly glycosidic prompt fragmentation (glycosidic C-cleavages and desulfation). Non-sulfated aldoses and alditols, which could only be analyzed in positive-ion mode ([M+Na](+)), did not suffer any prompt fragmentation. The former yielded cross-ring fragmentation in the PSD mode. Best results were obtained by using 2,5-dihydroxybenzoic acid and/or nor-harmane as matrices for all the compounds studied. (C) 2009 Elsevier Ltd. All rights reserved.
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