Journal
CARBOHYDRATE RESEARCH
Volume 345, Issue 15, Pages 2124-2132Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2010.07.027
Keywords
Cyclodextran; Periodate oxidation; Reductive amination; Lectin
Funding
- Okinawa Prefecture, Japan
- Tropical Technology Center Ltd
Ask authors/readers for more resources
The synthesis of small glycoclusters with high affinity toward lectins is one of the important subjects in glycotechnology. Although cyclic alpha-(1 -> 6)-D-octaglucoside (CI8) is an attractive scaffold on which to put glycosyl pendants, the compound has only secondary hydroxyl groups, which are relatively unreactive for substitution reactions. The oxidation of the vicinal diols of CI8 and reductive amination of the resultant dialdehydes with 2-aminoethyl mannoside gave mannose-CI8 conjugates with a variety of average mannose incorporation numbers (2-7). The average numbers were deduced from MALDI-TOF mass and H-1 NMR spectroscopy. The binding ability of mannose-CI8 conjugates to concanavalin A increased with the increasing numbers of average mannose incorporation, reaching a plateau at tetravalence, as estimated from a latex bead-based agglutination lectin assay. Toxicity tests demonstrated the biocompatibility of mannose-CI8 conjugates. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available