Journal
CARBOHYDRATE RESEARCH
Volume 344, Issue 14, Pages 1858-1862Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2008.11.010
Keywords
Homogalacturonans; Pectic polysaccharides; Monoclonal antibodies; Plant cell wall
Funding
- UK Biotechnology and Biological Sciences Research Council
- EU [RTN CT-2004-512265, 517036]
- BBSRC [BB/D00098X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D00098X/1] Funding Source: researchfish
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Three novel rat monoclonal antibodies, designated LM18, LM19 and LM20, were isolated from screens for binding to Arabidopsis thaliana seed coat mucilage. The binding of these antibodies to mucilage subject to enzyme and high pH pre-treatments and to a series of model homogalacturonan-rich pectins with defined levels of methyl-esterification indicated their recognition of pectic homogalacturonan epitopes. The binding capacities of these monoclonal antibodies to cell walls in sections of tobacco stem pith parenchyma were also differentially sensitive to equivalent treatments with high pH buffers and pectate lyase. The epitopes bound by these antibodies display some similarities and some differences to the epitopes recognized by the previously isolated and established pectic homogalacturonan probes JIM5 and JIM7. (C) 2008 Elsevier Ltd. All rights reserved.
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