Journal
CARBOHYDRATE RESEARCH
Volume 344, Issue 14, Pages 1798-1807Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2008.08.036
Keywords
Homogalacturonan; Rhamnogalacturonan; Pectic oligosaccharides; Cell wall polysaccharides
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Pectin are extremely complex biopolymers made up of different structural domains. Enzymatic degradation followed by purification and structural analysis of the degradation products proved to be efficient tools for the understanding of pectin fine structure, including covalent interactions between pectic structural domains or with other cell wall polysaccharides. Due to its high sensitivity, high throughput and capacity to analyze mixtures, mass spectrometry has gained more and more importance as a tool for oligosaccharides structural characterization in the past 10 years. This review will focus on the combined use of mass spectrometry and enzymatic digestion for pectins structural characterization. (C) 2008 Elsevier Ltd. All rights reserved.
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