Journal
CARBOHYDRATE RESEARCH
Volume 344, Issue 13, Pages 1612-1619Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2009.04.019
Keywords
Amylosucrase; Deinococcus geothermalis; Glucosyl-salicin; Maltosyl-salicin; Salicin; Transglycosylation
Funding
- Korean Government (MOEHRD) [KRF-2006-311-F00025]
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Amylosucrase (ASase, EC 2.4.1.4) is a member of family 13 of the glycoside hydrolases; that catalyze the synthesis of an alpha-(1 -> 4)-linked glucan polymer from sucrose instead of an expensive activated sugar, such as ADP- or UDP-glucose. Transglycosylation reactions mediated by the ASases of Deinococcus geothermalis (DGAS) and Neisseria polysaccharea (NPAS) were applied to the synthesis of salicin glycosides with sucrose serving as the glucopyranosyl donor and salicin as the acceptor molecule. Two salicin glycoside transfer products were detected by TLC and HPLC analyses. The synthesis of salicin glycosides was very efficient with NPAS with a yield of over 90%. In contrast, DGAS specifically synthesized only one salicin ransglycosylation product. The transglycosylation products were identified as alpha-D-glucopyranosyl-(1 -> 4)-salicin (glucosyl salicin) and alpha-D-glucopyranosyl-(1 -> 4)-alpha-D-glucopyranosyl-(1 -> 4)-salicin (maltosyl salicin) by NMR analysis. The ratio between donor and acceptor had a significant effect on the type of product that resulted from the transglycosylation reaction. With more acceptors present in the reaction, more glucosyl salicin and less maltosyl salicin were synthesized. (C) 2009 Elsevier Ltd. All rights reserved.
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