Journal
CARBOHYDRATE RESEARCH
Volume 343, Issue 7, Pages 1171-1179Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2008.03.018
Keywords
pseudomonas; chitinase; chitosanase; shrimp shell wastes
Ask authors/readers for more resources
A chitinase (CHT1) and a chitosanase (CHS1) were purified from the culture supernatant of Pseudomonas sp. TKU015 with shrimp shell wastes as the sole carbon and nitrogen source. The optimized conditions of this new species strain (Gen Bank Accession Number EU103629) for the production of chitinases were found to be when the culture was shaken at 30 degrees C for 3 days in 100mL of medium (PH 8) containing 0.5% shrimp shell powder (SSP) (w/v), 0.1% K2HPO4, and 0.05% MgSO4 center dot 7H(2)O. The molecular weights of CHT1 and CHS1 determined by SDS-PAGE were approximately 68 kDa and 30 kDa, respectively. The optimum PH, optimum temperature, PH stability, and the thermal stability of CHT1 and CHS1 were PH 6, 50 degrees C, PH 5-7, < 50 degrees C and PH 4, 50 degrees C, PH 3-9, < 50 degrees C, respectively. CHT1 was inhibited completely by Mn2+ and Fe2+, and CHS1 was inhibited by Mn2+, CU2+, and PMSF. CHT1 was only specific to chitin substrates, whereas the relative activity of CHSI increased when the degree of deacetylation of soluble chitosan increased. (c) 2008 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available