Journal
CARBOHYDRATE RESEARCH
Volume 343, Issue 6, Pages 1041-1049Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2008.02.021
Keywords
rhamnogalacturonan; enzymatic degradation; oligosaccharide; pectin; Gossypium hirsutum
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An alkali extract of cell walls of Gossypium hirsutum L. was sequentially digested by endo-polygalacturonase (EC 3.2.1.15), arabinofuranosidase AN1571.2 (EC 3.2.1.55), endo-arabinase (EC 3.2.1.99), and rhamnogalacturonan hydrolase AN9314.2 (EC 3.2.1.15). The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, size-exclusion, and anion exchange chromatography. Fractions from the anion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOFMS. A new oligosaccharide (RGS29), which contained a rhamnogalacturonan dimer backbone with two galactose and two arabinose residues in the side chains, was found. Its structure was identified by 1D and 2D NMR spectra as follows: [GRAPHICS] (c) 2008 Elsevier Ltd. All rights reserved.
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