Journal
CARBOHYDRATE POLYMERS
Volume 106, Issue -, Pages 393-402Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.01.041
Keywords
Jellyfish; Rhopilema esculetum; Polysaccharide; Foam cell; Atherosclerosis
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Funding
- National Natural Science Foundation of China [31271814, 211006019]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20130111110001]
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In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP). The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100 degrees C and extraction time 4h. Under these conditions, the JSP yield reached 1.007 mg/g. Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP. After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively. The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition. In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions. (C) 2014 Elsevier Ltd. All rights reserved.
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