Chemical characterization, antioxidant and antitumor activity of sulfated polysaccharide from Sargassum horneri

Three water-soluble polysaccharide fractions (SHP30, SHP60, and SHP80) extracted from the Sargassum horneri were obtained by water extraction and radial flow chromatography. The high-performance gel-permeation chromatography analysis showed that the average molecular weight (Mw) of three polysaccharides were approximately 1.58 x 10(3), 1.92 x 10(3) and 11.2 KDa, respectively. Their in vitro antioxidant activities, antitumor activities were investigated and compared. Among these three polysaccharides, SHP30 with the highest sulfate content and intermediate molecular weight exhibited excellent antioxidant and antitumor activities in the superoxide radical assay, hydroxyl radical assay, reducing power assay, and MU assay. Then, flow cytometry assay and quantitative real-time reverse transcription-PCR analysis suggested that the accumulation of cells in G(0)/G(1) and S phase effecting apoptosis-associated gene expressions such as Bcl-2 and Bax might account for the growth inhibition of DLD cells by SHP30. Based on these results, we have inferred that sulfate content and molecular weight were the factors influencing antioxidant and antitumor activities. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.