Journal
CARBOHYDRATE POLYMERS
Volume 105, Issue -, Pages 260-269Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.01.073
Keywords
Sargassum horneri; Sulfated polysaccharide; Antioxidant activity; Antitumor activity
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Funding
- National Natural Science Foundation of China [31301560]
- Young Talent Program of Zhejiang University of Technology
- Major Projects for Science and Technology Foundation of Zhejiang Province [2011C12040]
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Three water-soluble polysaccharide fractions (SHP30, SHP60, and SHP80) extracted from the Sargassum horneri were obtained by water extraction and radial flow chromatography. The high-performance gel-permeation chromatography analysis showed that the average molecular weight (Mw) of three polysaccharides were approximately 1.58 x 10(3), 1.92 x 10(3) and 11.2 KDa, respectively. Their in vitro antioxidant activities, antitumor activities were investigated and compared. Among these three polysaccharides, SHP30 with the highest sulfate content and intermediate molecular weight exhibited excellent antioxidant and antitumor activities in the superoxide radical assay, hydroxyl radical assay, reducing power assay, and MU assay. Then, flow cytometry assay and quantitative real-time reverse transcription-PCR analysis suggested that the accumulation of cells in G(0)/G(1) and S phase effecting apoptosis-associated gene expressions such as Bcl-2 and Bax might account for the growth inhibition of DLD cells by SHP30. Based on these results, we have inferred that sulfate content and molecular weight were the factors influencing antioxidant and antitumor activities. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved.
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