Journal
CARBOHYDRATE POLYMERS
Volume 104, Issue -, Pages 191-198Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.01.024
Keywords
Cell-laden beads; Alginate; Tissue regeneration; 3D scaffold; Bone
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Funding
- National Research Foundation of Korea
- Ministry of Education, Science, and Technology (MEST) [NRF-2012R1A2A2A01017435]
- Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [A120942]
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We propose a new cell-encapsulated dispensing method consisting of hydrogel struts, embedded with cell-laden micro-beads. To develop the scaffolds, we accommodated a three-axis robot dispensing system and aerosol spraying of a cross-linking agent to effect tentative surface gelation of hydrogel alginate struts. To show the feasibility of the method, we used pre-osteoblast (MC3T3-E1) cells. Using this technique, we obtained a reasonable cell viability (>90% after several culture periods) relative to that of a scaffold onto which cells were dispensed in the conventional manner, and successfully fabricated a realistic macroscopic pore-size in a controlled manner with 100% pore-interconnected 3D alginate hydrogel scaffolds of 20 mm x 20 mm x 6 mm. (C) 2014 Elsevier Ltd. All rights reserved.
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