Journal
CARBOHYDRATE POLYMERS
Volume 110, Issue -, Pages 142-147Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.03.060
Keywords
Lonicera japonica Thunb.; A beta(42); Glucan; Aggregation and neurotoxicity; Alzheimer's disease
Categories
Funding
- New Drug Creation and Manufacturing Program [2012ZX09301001-003]
- National Science Fund [81125025]
- National Natural Science Foundation of China [31230022]
- Industry-University-Research Institute Alliance Fund in Guangdong Province, China [2010A090200041]
- Shanghai Research Program [13ZR1447500]
Ask authors/readers for more resources
Inhibition of A beta aggregation and attenuation of its cytotoxicity are considered to valuable therapeutics for Alzheimer's disease (AD). Here, a glucan named as LJW0F2 was purified from flowers of Lonicera japonica Thunb. Using monosaccharides composition analysis, methylation analysis, IR and NMR spectroscopy, this polysaccharide was elucidated to be an alpha-D-(1 -> 4)-glucan with an alpha-(1 -> 4) linked branch attached to the C-6 position. Its inhibitory effect on A beta(42) aggregation was measured by fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM). We showed that polysaccharide LJW0F2 could inhibit A beta(42) aggregation in a dose-dependent-manner. Besides, LJW0F2 could attenuate the cytotoxicity induced by A beta(42) aggregation in SH-SY5Y neuroblastoma cells. To the best of our knowledge, this was the first report that the exogenous plant-derived polysaccharide might block A beta(42) aggregation directly and reduce its toxicity in SH-SY5Y cells. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available