Journal
CARBOHYDRATE POLYMERS
Volume 113, Issue -, Pages 380-387Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.07.027
Keywords
Ultrasonic treatment; Phellinus igniarius; Polysaccharides; Response surface methodology; Chemical property; Antioxidant activity
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Funding
- National Natural Science Foundation of China [31271966]
- National Science and Technology Support Program [2012BAD36B05]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
- Science and Technology Support Plan (Industry) of Zhenjiang [GY2013006]
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In this study, a novel flat-plate ultrasound technology was developed to stimulate polysaccharides production from Phellinus igniarius mycelial fermentation. Three-factor-three-level Box-Behnken design was used to optimize ultrasonic treatment time, duty cycle time and culture time for a high yield of P. igniarius polysaccharides (PIPS). Optimal conditions were found to be ultrasound treatment time 65 min, duty cycle time 25s, and culture time 3.8 d that gave a maximum PIPS yield of 1.8002 g/L, which increased similar to 22.64% compared with the control (without any ultrasound). PIPS mainly contained low-molecular weight (MW) polysaccharides (3.1 kDa, 80%) composed of glucose, rhamnose and mannose in a molar ratio of 11.0:14.0:1.0. PIPS with higher carbohydrate and uronic acid contents exhibited strong antioxidant activities in vitro. Laser scanning confocal microscope (LSCM) observations suggested that ultrasound could change the morphology and structure of P. igrtiarius mycelium, and accelerate the transfer of nutrients and metabolites. (C) 2014 Elsevier Ltd. All rights reserved.
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