Journal
CARBOHYDRATE POLYMERS
Volume 108, Issue -, Pages 331-337Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.02.053
Keywords
Chitosanase; Squid pen; Penicillium janthinellum; Chitooligomers; Chitosanase activator; Boron
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Funding
- National Science Council Taiwan [NSC 102-2313-B-032-001-MY3, NSC 102-2621-M-032-005, NSC 102-2811-B-032-001]
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Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7-9, 60 degrees C and was stable at pH 7-11, 25-50 degrees C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains. (C) 2014 Elsevier Ltd. All rights reserved.
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