Journal
CARBOHYDRATE POLYMERS
Volume 113, Issue -, Pages 217-224Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.07.015
Keywords
Polyelectrolyte complexes; DEAE Dextran; ITC; Polysaccharide
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Funding
- CNRS (France) [23720]
- Romanian Academy of Science [23720]
- French Ministere de l'Education Nationale, de la Recherche et de la Technologie
- Region Haute-Normandie
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The formation of polyelectrolyte complexes (PECs) between carboxymethyl pullulan and DEAE Dextran, was investigated, in dilute solution, with emphasis on the effect of charge density (molar ratio or pH) and molar masses. Electrophoretic mobility measurements have evidenced that insoluble PECs (neutral electrophoretic mobility) occurs for charge ratio between 0.6 (excess of polycation) and 1 (stoichiometry usual value) according to the pH. This atypical result is explained by the inaccessibility of some permanent cationic charge when screened by pH dependant cationic ones (due to the Hoffman alkylation). Isothermal titration calorimetry (ITC) indicates an endothermic formation of PEC with a binding constant around 10(5) L mol(-1). Finally asymmetrical flow field flow fractionation coupled on line with static multi angle light scattering (AF4/MALS) evidences soluble PECs with very large average molar masses and size around 100 nm, in agreement with scrambled eggs multi-association between various polyelectrolyte chains. (C) 2014 Elsevier Ltd. All rights reserved.
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