Journal
CARBOHYDRATE POLYMERS
Volume 111, Issue -, Pages 908-917Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.05.057
Keywords
O-Urocanyl pullulan; pH-sensitive; Nanoparticle carrier; Drug resistance; Cancer
Categories
Funding
- 973 Program [2011CB933100]
- National Natural Science Foundation of China [81371671, 81201646]
- special program of China Postdoctoral Science Foundation [201104308]
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Urocanic acid was conjugated to pullulan to synthesize O-urocanyl pullulan (URPA) with degree of substitution (DS) of 8.2%. URPA nanoparticles prepared by dialysis method had spherical shapes and a mean diameter of 156.8 +/- 16.8 nm. Adriamycin (ADR) was successfully loaded into URPA nanoparticles and exhibited pH-sensitive in vitro release property. KIT assay showed that ADR-loaded URPA (ADR/URPA) nanoparticles had a significant higher toxicity against drug resistant MCF-7/ADR cells than free ADR, and the reversal index reached up to 9.6. The results of flow cytometry and confocal microscopy showed that URPA nanoparticles efficiently enhanced accumulation and retention of ADR in MCF-7/ADR cells and successfully delivered ADR into cell nucleus. The reversal effect of ADR/URPA nanoparticles on the drug resistance of MCF-7/ADR cells was perhaps related with their cell entry and intracellular drug release mechanisms. (C) 2014 Elsevier Ltd. All rights reserved.
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