Journal
CARBOHYDRATE POLYMERS
Volume 102, Issue -, Pages 238-245Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.11.040
Keywords
Green synthesis; Silver nanoparticles; Schizophyllan; Cytotoxicity
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Funding
- Deanship of Scientific Research at King Saud University [RGP-VPP-201]
- project CEITEC - Central European Institute of Technology - excellent teams [CZ.1.07/2.3.00/30.0005, CZ.1.05/1.1.00/02.0068]
- European Social Fund
- European Regional Development Fund
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Silver nanoparticles (Ag-NPs) have been successfully prepared with a simple and green chemical reduction method. Triple helical schizophyllan (SPG) was used for the first time as reducing and stabilizing agents. The effect of temperature, silver nitrate/schizophyllan concentrations, pH of the reactions medium and the reaction time were investigated. The obtained schizophyllan/Ag-NP was characterized by UV-vis spectroscopy, TEM, DLS, X-ray diffraction, TGA, and ATR-FTIR. The results revealed that, Ag-NPs attached to SPG through a strong non-covalent interaction, leading to good dispersion of Ag-NPs with a diameter of 6 nm within the biopolymer matrix. By increasing the pH of the reaction medium, the triple helical structure of SPG was partially broken. The SPG/AgNP nanocomposite was non-toxic for mouse fibroblast line (NIH-3T3) and human keratinocyte cell line (HaCaT). (C) 2013 Elsevier Ltd. All rights reserved.
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