Journal
CARBOHYDRATE POLYMERS
Volume 101, Issue -, Pages 606-613Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.09.099
Keywords
Hericium erinaceus polysaccharides; Enzyme-assisted extraction; Response surface methodology; Optimization; Characterization
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Funding
- Practice Innovative Projects in China [2012JSSPITP1240]
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The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03 degrees C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 +/- 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes. (C) 2013 Elsevier Ltd. All rights reserved.
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