Journal
CARBOHYDRATE POLYMERS
Volume 110, Issue -, Pages 278-284Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.03.040
Keywords
Lycium barbarum polysaccharides; Ultrasound-assisted enzymatic extraction; Response surface methodology; Optimization
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Funding
- National Natural Science Foundation of China [41373114, 41201487]
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In this study, an efficient ultrasound-assisted enzymatic extraction procedure for the water-soluble polysaccharides from the fruit of Lycium barbarum was investigated and optimized. Response surface methodology (RSM) based on a three-level four-factor Box Behnken Design (BBD) was employed to optimize the extraction conditions inlcluding extraction time, ultrasonic output power, cellulose concentration and extraction temperature. The experimental data were adequately fitted into a second-order polynomial model. The optimized conditions were as follows: extraction time 20.29 min, ultrasonic output power 78.6W, cellulose concentration 2.15%, extraction temperature 55.79 degrees C. Under these conditions, the experimental yield of polysaccharides was 6.31 +/- 0.03%, which matched with the predictive yield of 6.32% well. (C) 2014 Elsevier Ltd. All rights reserved.
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