Journal
CARBOHYDRATE POLYMERS
Volume 98, Issue 2, Pages 1524-1531Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.07.052
Keywords
Flammulina velutipes; Antioxidant activity; Polysaccharide; Extraction method
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Funding
- Great Project of Science Technology Department of Zhejiang Province [2011C12046]
- Project of Innovation and Promotion of Zhejiang Academy of Agriculture Science
- Project of Science and Technology of Jiaxing, Zhejiang Province [2012AY1058]
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Four polysaccharides (CFP, UFP, MFP and EFP) were extracted from Flammulina velutipes using hot water, ultrasonic, microwave or enzymatic methods optimized by orthogonal test. Preliminary structural characterizations were conducted using physicochemical properties. Polysaccharides extracted by all four methods showed similar physicochemical characteristics and FT-IR spectra. However, SEM images of tissues of F. velutipes were significantly different. EFP demonstrated better antioxidant activities against hydroxyl radical as well as improved metal chelating activity. UFP showed higher DPPH scavenging activity, but CFP exhibited higher antioxidant activity in reducing power. Hence, these polysaccharides can be used as natural antioxidants in functional foods or medicine. Further experiments on the biological activities of these four polysaccharides are currently in progress. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
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