Journal
CARBOHYDRATE POLYMERS
Volume 91, Issue 1, Pages 128-134Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.08.012
Keywords
Bifidobacterium animalis; Exopolysaccharides; Purification; Structure
Categories
Funding
- Beijing Natural Science Foundation [5122018]
- Program of Higher-level talents of Inner Mongolia University (SPH-IMU) [115114]
Ask authors/readers for more resources
An exopolysaccharide fraction (EPSb) produced by Bifidobacterium animalis RH isolated from the feces of centenarians was purified to illustrate its structure and conformational characterization. Results from Fourier-transform infrared spectrometry, gel permeation chromatography, high-pH anion-exchange chromatography with pulsed amperometric detection, periodate oxidation and Smith degradation, methylation and gas chromatography-mass spectrometry, and nuclear magnetic response analysis indicated that EPSb (M-w = 21.3 kDa) was composed of rhamnose (Rha), arabinose (Ara), galactose (Gal), glucose (Glc), and mannose (Man) in a molar ratio of 0.4:0.3:1.6:0.8:1.2. This compound had a backbone of (1 -> 3,4)-linked Man, (1 -> 4)-linked Rha, (1 -> 4)-linked Gal, and (1 -> 4)-linked Glc. It was branched with Gal and terminated with Gal and Glc residues. The molecular structure of EPSb was observed via atomic force microscopy. EPSb showed spherical lumps and a ring-like network. Conformational analysis revealed the non-triple helical conformation of EPSb. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available