Journal
CARBOHYDRATE POLYMERS
Volume 92, Issue 1, Pages 604-611Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.09.020
Keywords
Ultrasound; Polysaccharide; Corn silk; Box-Behnken design; Extraction
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In this study, ultrasound assisted extraction (UAE) conditions on the yield of polysaccharide from corn silk were studied using three factors, three level Box-Behnken response surface design. Process parameters, which affect the efficiency of UAE such as extraction temperature (40-60 degrees C), time (10-30 min) and solid-liquid ratio (1:10-1:30g/ml) were investigated. The results showed that, the extraction conditions have significant effects on extraction yield of polysaccharide. The obtained experimental data were fitted to a second-order polynomial equation using multiple regression analysis with high coefficient of determination value (R-2) of 0.994. An optimization study using Derringer's desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variables (extraction temperature of 56 degrees C, time of 17 min and solid-liquid ratio of 1:20 g/ml) were determined with maximum polysaccharide yield of 6.06%, which was confirmed through validation experiments. (C) 2012 Elsevier Ltd. All rights reserved.
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