Journal
CARBOHYDRATE POLYMERS
Volume 98, Issue 1, Pages 108-115Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.04.050
Keywords
Mauran; Extremophiles; Oxidative stress; Antioxidant; Antithrombogenic; Antihemolytic; Halomonas; Polysaccharides
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- grant for the programme of the strategic research foundation at private universities [S1101017]
- Japan Society for the Promotion of Science (JSPS)
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Mauran (MR), a highly polyanionic sulfated exopolysaccharide was extracted from moderately halophilic bacterium; Halomonas maura and characterized using X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Purified MR was evaluated for antioxidant defense mechanisms under in vitro conditions using L929, mouse fibroblast cell line and mice liver homogenate. It was demonstrated that MR could impart protective effect against oxidative stress in both cells and tissue up to a concentration of 500 mu g, which is found to be safe under laboratory conditions. Various enzymatic and non-enzymatic parameters of antioxidant mechanisms were evaluated and concluded that MR has the tendency to maintain a balance of antioxidative enzymes with in the test systems studied. Also, hemocompatibility assay performed revealed that MR has a lesser hemolytic index and exhibited a prolonged clotting time, which shows both antihemolytic, and antithrombogenic nature respectively. Furthermore, absorption studies performed using fluorescent-labeled MR confirmed that MR accumulated within the cell cytoplasm neither induced cellular lysis nor affected the cell integrity. (C) 2013 Elsevier Ltd. All rights reserved.
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