Statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed

The purpose of the work was to optimize the medium variables for maximizing pullulan production using jack fruit seed as a low cost substrate by Aureobasidium pullulans in solid state fermentation. Effects of K2HPO4, KH2PO4, ZnSO4 center dot 5H(2)O, MgSO4 center dot 7H(2)O, NaCl, (NH4)(2)SO4 center dot 5H(2)O, yeast extract, moisture content (%, w/w) in the production medium on pullulan production were studied using Plackett-Burman design. Production of pullulan was significantly affected by the medium variables namely KH2PO4, ZnSO4 center dot 5H(2)O, NaCl and moisture content (%, w/w).Then screened variables were optimized by Box Behnken experiment design. The pullulan obtained was characterized and confirmed by FTIR, H-1 NMR and C-13 NMR. Molecular weight of pullulan was found to be 1.733 x 10(6) g/mol by gel permeation chromatography (GPC). (C) 2013 Elsevier Ltd. All rights reserved.