Journal
BIOINORGANIC CHEMISTRY AND APPLICATIONS
Volume 3, Issue 3-4, Pages 239-253Publisher
HINDAWI LTD
DOI: 10.1155/BCA.2005.239
Keywords
gold(III) complexes; platinum(II) complexes; calf thymus DNA (ct-DNA); pHV14 plasmid; circular dichroism; absorption spectroscopy; DNA melting; ultrafiltration
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The interactions of the parent complexes [AuCl(Terpy)]Cl-2 and [PtCl(Terpy)]Cl with DNA were analysed by various physicochemical methods. Surprisingly, these metal complexes produce different interaction patterns with DNA in spite of their profound structural similarity. Indeed, important modifications are detected in the characteristic UV-Vis bands of [PtCl(Terpy)]Cl upon addition of ct-DNA, while the spectrum of [AuCl(Terpy)]Cl-2 is almost unaffected. Gel electrophoresis studies confirm these findings: [PtCl(Terpy)]Cl - but not [AuCl(Terpy)]Cl-2 - retards significantly the mobility of the supercoiled form of the pHV14 plasmid after a short incubation time. Ultrafiltration studies indicate that the affinity of [PtCl(Terpy)]Cl for ct-DNA is significantly greater than that of [AuCl(Terpy)]Cl-2. On the other hand, both [AuCl(Terpy)]Cl-2 and [PtCl(Terpy)]Cl induce important changes in the CD spectrum of ct-DNA, at high concentration, and increase its T-m value. Remarkably, the analysed metal-complex/DNA interaction patterns depend critically on the incubation times. We propose that [PtCl(Terpy)]Cl quickly intercalates DNA; then, formation of coordinative bonds progressively takes place with time. At variance, [AuCl(Terpy)]Cl-2 first interacts electrostatically with the DNA surface, with subsequent slow formation of some coordinative bonds.
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