Polysaccharide extraction from Abelmoschus esculentus: Optimization by response surface methodology

Crude polysaccharide extraction from the Iranian Abelmoschus esculentus was performed using water decoction. Response surface methodology (RSM), based on a five level, four variable central composite rotatable design (CCRD), was employed to obtain the best possible combination of extraction time (X-1 : 0.5-6.5 h), extraction temperature (X-2: 80-100 degrees C), number of extraction (X-3: 1-5), and water to the raw material ratio (X-4: 4-28) for maximum polysaccharide extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: extraction time of 4.94 h, extraction temperature of 94.97 degrees C, number of extraction of 4, and the ratio of water to raw material of 21.74. Under these conditions, the experimental yield was 16.895 +/- 0.29%, which is well in close agreement with the value predicted by the model 16.916%. (c) 2013 Elsevier Ltd. All rights reserved.