Journal
CARBOHYDRATE POLYMERS
Volume 98, Issue 1, Pages 976-981Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.07.011
Keywords
Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharide; High-performance anion-exchange chromatography; Monosaccharide composition
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Funding
- Program of the National Natural Science Foundation of China [31201297, 31130041]
- Natural Science Foundation for Young Scientists of Jiangxi Province, China [20122BAB214004]
- National Key Technology R & D Program of China [2012BAD33B06]
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A simple, rapid and sensitive analytical method for the determination of monosaccharide composition in Cyclocarya paliurus polysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC (TM) PA10 analytical column. The established method was validated and the results showed that this method had good linearity (R-2,0.9987-0.9999), adequate accuracy (98.53-102.13% recovery), high precision (relative standard deviation <3.8%) and sensitive detection limits (2.57-7.86 nM), with a simple preparation of the samples (no need to derivatize the samples) and short run time (20 min). The results showed that the C paliurus polysaccharide consisted of rhamnose, arabinose, galactose, glucose, mannose and xylose in the molar ratio of 1.00:1.85:3.26:3.12:0.85:0.29. (C) 2013 Elsevier Ltd. All rights reserved.
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