Journal
CARBOHYDRATE POLYMERS
Volume 98, Issue 2, Pages 1353-1359Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.07.075
Keywords
Verticillium lecanii; Lecanicillium lecanii; Glucogalactan; NMR spectroscopy
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Funding
- FAPESP [05/53879-3, 2010/05567-0]
- SETI/Fundacao Araucaria [14669]
- MCT/CNPq (Pronex/Carboidratos)
- CNPq [145852/2010-5]
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A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and ID and 2D H-1 and C-13 NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (EaSiv fraction) was established: [GRAPHICS] where n approximate to 22 and m approximate to 22. (C) 2013 Elsevier Ltd. All rights reserved.
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