Glucogalactan: A polysaccharide isolated from the cell-wall of Verticillium Lecanii

A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and ID and 2D H-1 and C-13 NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (EaSiv fraction) was established: [GRAPHICS] where n approximate to 22 and m approximate to 22. (C) 2013 Elsevier Ltd. All rights reserved.