Journal
CARBOHYDRATE POLYMERS
Volume 92, Issue 2, Pages 1908-1914Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.10.034
Keywords
beta-(1 -> 6)-D-Glucan; Lasiodiplodia theobromae; Lasiodiplodan; Sulfonation; Anticoagulant activity
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Funding
- CAPES (Brazil)
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An exocellular beta-(1 -> 6)-D-glucan (lasiodiplodan) produced by a strain of Lasiodiplodia theobromae (MMLR) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. The structural features of the sulfonated beta-(1 -> 6)-D-glucan were investigated by UV-vis, FT-IR and C-13 NMR spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays APTT, PT and TT using heparin as standard. The content of sulfur and degree of substitution of the sulfonated glucan was 11.73% and 0.95, respectively. UV spectroscopy showed a band at 261 nm due to the unsaturated bond formed in the sulfonation reaction. Results of FT-IR and C-13 NMR indicated that sulfonyl groups were inserted on the polysaccharide. The sulfonated beta-(1 -> 6)-D-glucan presented anticoagulant activity as demonstrated by the increase in dose dependence of APTT and TT, and these actions most likely occurred because of the inserted sulfonate groups on the polysaccharide. The lasiodiplodan did not inhibit the coagulation tests. (C) 2012 Elsevier Ltd. All rights reserved.
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