Journal
CARBOHYDRATE POLYMERS
Volume 89, Issue 1, Pages 17-23Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.01.093
Keywords
Funalia trogii; Exopolysaccharides; Mycelial biomass; Optimization; Antioxidant; Antitumor
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Optimization of culture conditions for exopolysaccharide (EPS) by Funalia trogii in submerged culture was investigated using one-factor-at-a-time method and uniform design (UD). Under the optimized conditions, the maximum concentration of EPS was 8.68 g/l. After EPS was deproteinized by Sevag method, two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by gel filtration chromatography on Sepharose CL-6B. Furthermore, EPSs were characterized by size exclusion chromatography (SEC) coupled with a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar masses of the Fr-I and Fr-II were determined to be 1.007 x 10(5) and 2.393 x 10(4) g/mol, respectively. The root mean square (RMS) radii for both peaks ranged from 9.7 to 10.8 nm with no clear trends. Pharmacology experiments indicated F. trogii EPS were useful to the therapy of free radical injury and cancer diseases. (C) 2012 Elsevier Ltd. All rights reserved.
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