Journal
CARBOHYDRATE POLYMERS
Volume 89, Issue 2, Pages 519-525Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.03.039
Keywords
Ginseng polysaccharide; Arabinogalactan; RG-I domain; Partial acidic hydrolysis; Immunological activity
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Funding
- National Natural Science Foundation of China [31170770, 30973857, 81173605]
- Fundamental Research Funds for the Central Universities [09SSXT125, 10SSXT146, 10QNJJ012]
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In this paper, we further analysed the structure of a type I rhamnogalacturonan (RG-I) pectin (WGPA-2-RG) fractionated from ginseng polysaccharides. Methylation and periodate oxidation analyses showed that WGPA-2-RG has a backbone consisting of alternating rhamnose (Rha) and galacturonic acid (GalA) residues and side chains consisting of type II arabinogalactan (AG-II). Partial acidic hydrolysis for 6h completely removed arabinose (Ara), partial galactose (Gal), but little GalA and Rha. During partial hydrolysis, the molecular weight of WGPA-2-RG decreased smoothly, suggesting that the Ara and cleavable Gal residues exist on the surface of the molecule, while GalA and Rha residues exist in the core of the molecule. The bioactivity assay showed that the arabinogalactan side chains of WGPA-2-RG are essential structures for stimulating NO secretion and lymphocyte proliferation. However, removal of the Ara and Gal residues through hydrolysis did not appreciably affect the ability of WGPA-2-RG to enhance macrophage phagocytosis. (C) 2012 Elsevier Ltd. All rights reserved.
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