Journal
CARBOHYDRATE POLYMERS
Volume 88, Issue 1, Pages 103-111Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2011.11.072
Keywords
Starch; Amylose; Amylopectin; Chromatography; GPC; Iodine colorimetry; Chain-length distribution
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Funding
- Australian Research Council [DP0985694]
- Knut and Alice Wallenberg Foundation (Sweden)
- Australian Research Council [DP0985694] Funding Source: Australian Research Council
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A new analytical method to define and quantify the amylose content in starches is developed using two-dimensional (2D) macromolecular size/branch chain-length distributions obtained by multidimensional size-exclusion chromatography (SEC, also known as GPC) and enzymatic debranching. This method permits clear separation of amylose (low molecular weights, a small number of long-chain branches), amylopectin (high molecular weights, a large number of short-chain branches), long-chain-branched amylopectin and intermediate components. The results are applied to rice starch, normal maize starch, and two high-amylose starches (Gelose 50 and Gelose 80) and used to validate four single-quantity techniques for measuring amylose content: iodine colorimetry, concanavalin A precipitation, and 10 SEC debranched (or chain-length) and branched distributions. Quantitatively accurate amylose contents can be obtained with the first three single-quantity methods for starch samples with clear separation of the amylose and amylopectin populations, but the 10 SEC branched distribution seriously overestimates the values compared to the other techniques. For high-amylose starches, the definition of amylose content must be taken with caution: it is impossible to separate the different macromolecular populations unambiguously because of the higher abundance of hybrid species. The 2D structural method serves as a reference to identify amylose content and validate single-quantity analytical procedures. (C) 2011 Elsevier Ltd. All rights reserved.
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