Journal
CARBOHYDRATE POLYMERS
Volume 89, Issue 2, Pages 542-550Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.03.042
Keywords
Collagen; Chondroitin sulfate; Hydrogen bond; Electrostatic interaction; Two-dimensional infrared correlation spectroscopy (2D IR)
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Funding
- National Natural Science Foundation of China [21076129/B060805]
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The neutral homogeneous collagen/chondroitin sulfate (Col/CS) blends were prepared when using 0.25 mol/LNaCl to stabilize the blends. Composition-dependent Fourier transform-infrared (FTIR) spectra were analyzed by generalized two-dimensional (2D) correlation spectroscopy to investigate the conformational changes of collagen and the specific interactions between collagen and CS. When adding CS to the collagen solution, C-H bending vibration of collagen varies firstly, then collagen skeleton, and finally the specific interactions between collagen and CS. The Col/CS ratios influence the kinds of specific interactions. Only hydroxyl groups of CS interact with C=O group of collagen owing to the charge shielding effect of NaCl when the CS content is less than 50 wt%. Besides hydrogen bond, the electrostatic interactions between ionized carboxyl group or sulfate group of CS and E-amino group of lysine or guanidine group of arginine appear when the CS content is more than 50 wt%. (C) 2012 Elsevier Ltd. All rights reserved.
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