Characterization and functional elucidation of a fucosylated 1,6-α-D-mannogalactan polysaccharide from Antrodia cinnamomea

Antrodia cinnamomea is a valuable polyporaceous edible fungus native to Taiwan It was reported to provide a number of pharmaceutical benefits A. cinnamomea was cultured the polysaccharides (PSs) were extracted and chromatographically fractionated and their biological functions were evaluated The PS subtractions (B85PS-I-V) showed differential inhibition of in vitro Matrigel tube formation (an indicator of angiogenesis inhibition) with IC50 values of 7 44 16 41 707 798 and 16 33 mu g/ml respectively Furthermore A. cinnamomea PSs also blocked vascular endothelial growth factor (VEGF)-induced endothelial cell migration B85PS-III and -V dose-dependently decreased angiogenic-related protein expressions including inhibition of VEGF receptor (VEGFR) phosphorylation and angiopoietin-2 protein expression To further purify and determine the structure of the bioactive PS B85PS-III was chromatographically purified to give a water-soluble partial fucosylated 1 6-alpha-d-mannogalactan (B85PS-III-1) composed of a nonadecasaccharide repeating unit with a molecular mass of 4 17 x 10(2) kDa as shown below The chemical structure of B85PS-III-1 was characterized by a monosaccharide analysis along with H-1 C-13 and 2D nuclear magnetic resonance spectroscopy [GRAPHICS] (C) 2010 Elsevier Ltd All rights reserved