Journal
CARBOHYDRATE POLYMERS
Volume 84, Issue 3, Pages 1019-1026Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.12.061
Keywords
Exopolysaccharide; MALDI-TOF-TOF MS; Bacillus licheniformis; Seaweed; Atomic force microscopy; X-ray diffraction
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Funding
- Council of Scientific and Industrial Research, New Delhi [NWP-018]
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In the present study, EPS secreted by the endophytic bacterium Bacillus licheniformis was isolated and characterized. The molecular masses of the EPS were 1540 and 44,565 kDa and H-1 NMR, FT-IR and UV-Vis spectral analyses revealed prevalence of characteristic primary amine-group, aromatic-compound, halide and aliphatic alkyl-group in addition to Na, P, Ca, C, O, Cl and S as inferred from EDX analysis. XRD and DSC analysis confirmed the amorphous nature of EPS, showing an average particle size of 24.977 mu m (d 0.5) with 191 nm average roughness. The positive ion reflector mode of MALDI TOF-TOF MS exhibited a series of low and high mass peaks corresponding to various oligosaccharides and polysaccharides respectively. Further, GC-MS analysis revealed its four monosaccharide constituents glucose, galactose, mannose and arabinose. The potential heterogeneous properties of EPS as revealed in the present study may be explored in various biotechnological and industrial applications. (C) 2010 Elsevier Ltd. All rights reserved.
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