Isolation, purification and structure of a new water-soluble polysaccharide from Zizyphus jujuba cv Jinsixiaozao

A novel water-soluble polysaccharide (ZSP3c) was isolated from Zizyphus jujuba cv jinsixiaozao and its structure was investigated ZSP3c was obtained by extraction with hot water followed by purification using DEAE-SepharoseCL-6B anion-exchange SepharoseCL-6B and Sephadex G-200 column chromatography It was eluted as a single symmetrical narrow peak on high-performance gel-permeation chromatography (HPGPC) and the average molecular weight (Mw) was estimated to be 1 4 x 10(5) Da The ZSP3c was composed of L-rhamnose D-arabinose and o-galactose in a molar ratio of 1 2 8 The structural features of ZSP3c were investigated by partial hydrolysis with acid methylation analysis and NMR spectroscopy analysis The results revealed that the main backbone chain of ZSP3c was (1 -> 4)-D-galacturonopyranosyl residues interspersed with (1 -> 2)-L-rhamnopyranosyl residues and (1 -> 24)-L-rhamnopyranosyl residues The neutral side chains of arabinofuranosyl residues and galactopyranosyl residues were attached to the backbone at the O-4 position of rhamnopyranosyl residues (C) 2010 Elsevier Ltd All rights reserved