Journal
CARBOHYDRATE POLYMERS
Volume 86, Issue 1, Pages 120-126Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2011.04.017
Keywords
Biocompatibility; Carboxymethyl konjac glucomannan; Enzyme encapsulation; Nanospheres; Self-assembly
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Funding
- National Natural Science Foundation of China (NSFC) [21074138, 51073107, 50703025]
- State Key Laboratory of Polymer Materials Engineering (Sichuan University) [KF201003]
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The self-assembly of rod-coil carboxymethyl konjac glucomannan-graft-poly(ethylene glycol) (CKGM-g-PEG) and alpha-cyclodextrin (alpha-CD) complexes were investigated and used as encapsulating hollow nanospheres for the enzyme glucose oxidase (GOX) in aqueous solution. These hollow nanospheres exhibited cell-like semi-permeability allowing enzyme substrates to pass through the surface while restricting the encapsulated enzyme (i.e. GOX) to the interior. Encapsulated GOX exhibited higher thermostability, optimal enzymatic activity over a wider pH range and improved storage stability in comparison to free un-encapsulated GOX. In addition, these CKGM-g-PEG/alpha-CD hollow nanospheres showed in vitro biocompatibility when exposed to L929 cells when tested using MIT viability assay. These studies suggested that self-assembly of CKGM-g-PEG and alpha-CD to form stable nanospheres may be an effective method for enzyme encapsulation with numerous biomedical applications. (C) 2011 Elsevier Ltd. All rights reserved.
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