Effect of crosslinked condition on characteristics of chitosan/tripolyphosphate/genipin beads and their application in the selective adsorption of phytic acid from soybean whey

In this study, tripolyphosphate/genipin co-crosslinked chitosan beads were prepared in different pH solutions and applied for selective adsorption of phytic acid from soybean whey solution. The co-crosslinking degrees (81.20-59.22%) of beads decreased with increased pH value of solution. The major chemical linkage between chitosan and genipin and a little ionic interaction between chitosan and tripolyphosphate gave CB7 (co-crosslinked in pH 7 solution) the best mechanical strength among these beads. The best adsorption ratio of phytic acid of the co-crosslinked beads occurred in pH 1 solution. The adsorption ratios of CB7 for phytic acid, trypsin inhibitor and lectin from pH 2 soybean whey solution were 30.23%, 3.76% and 1.53%, respectively. The highest desorption ratio of phytic acid from the CB7 desorbed in pH 9 solution was 93.98%; selective isolation for phytic acid can definitely be processed by CB7. (C) 2011 Elsevier Ltd. All rights reserved.