Journal
CARBOHYDRATE POLYMERS
Volume 83, Issue 1, Pages 88-93Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.07.024
Keywords
Leuconostoc garlicum; Extracellular polysaccharide; Dextran; Structural analysis; FTIR and NMR spectroscopies
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Funding
- Czech Grant Agency [MSM 7088352101, GACR P108/10/0200]
- Slovak Grant Agency [VEGA 2/0155/08, APVV 0030-07]
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White and highly viscous extracellular polysaccharide composed of D-glucose residues has been isolated from culture medium of Leuconostoc garlicum PR by acetone precipitation followed by dialysis and freeze-drying. The crude biopolymer was recovered in the yield of 50 g/L of rich culture medium and showed an apparent molecular mass over 2 x 10(7). Chemical and spectroscopic studies revealed almost linear character of the bacterial biopolymer composed of alpha-1,6-linked D-glucopyranosyl backbone carrying a low content of branched points at C-2, C-3 and C-4. Moreover, a low content (similar to 2%) of alpha-1,4-linked D-glucopyranosyl residues has been determined in the extracellular biopolymer. (C) 2010 Elsevier Ltd. All rights reserved.
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