Journal
CARBOHYDRATE POLYMERS
Volume 80, Issue 2, Pages 344-349Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.11.028
Keywords
Phellinus igniarius; Response surface methodology; Extraction; Intracellular polysaccharide; Optimization experiment; Characterization
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Funding
- Chongqing Natural Science Foundation Program [2005881101]
- Opening Fund of Chongqing Key Laboratory of Luminescence and Real-time Analysis [2006CA8006]
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Polysaccharides of Phellinus igniarius have many bioactivities. In this study, response surface methodology along with Box-Behnken design based on the single-factor experiments was firstly applied to optimize the extraction conditions by its liquid-cultured mycelia. According to analysis, extraction temperature and ratio of mycelia to water significantly affected extraction yield. The optimal conditions were extraction temperature 70 degrees C, extraction time 1.5 h and the ratio of mycelia to water 1:6.2. Under these conditions, the maximal yield of crude intracellular polysaccharide (IPS) from mycelia was 50.39 +/- 0.41 mg/g, which was agreed with model predictions. The preliminary characterization was mainly B-galactan by gas chromatograph and infrared spectrum analysis. (c) 2009 Elsevier Ltd. All rights reserved.
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