Effects of sulfated lentinan on cellular infectivity of avian infectious bronchitis virus

In order to investigate the possibility of sulfating modification in improving the anti-viral activity of lentinan (LNT), three kinds of sulfated LNTs (sLNT), sLNT(1), sLNT(2) and sLNT(3) with the degree of substitution of 0.69, 0.98 and 1.37 were prepared by chlorosulfonic acid-pyridine method with three modification condition, respectively. The sLNTs and avain infectious bronchitis virus (IBV) were added into chicken embryo fibroblast (CEF) in three manners, pre-adding polysaccharide, post-adding polysaccharide and mixed adding polysaccharide with IBV, respectively, taking non-modified LNT as control. The anti-viral activities of three sLNTs were compared by MTT method. The results showed that all of sLNTs and LNT at a certain concentration could significantly inhibit IBV growth in three manners. The effects of modified sLNTs were better than that of non-modified LNT. It indicated that sulfated modification could enhance the anti-viral activity of LNT. (C) 2009 Elsevier Ltd. All rights reserved.