Journal
CARBOHYDRATE POLYMERS
Volume 82, Issue 3, Pages 848-853Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.06.006
Keywords
Polysaccharides; Opuntia ficus indica; DGlucan
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A water-soluble polysaccharide (PS-1) was isolated from the fruits of Opuntia ficus indica (L) Miller by hot water extraction, anion-exchange and gel-permeation chromatography (yield 167.5 mg/kg raw fruit; [alpha](D)(16 1) + 192 degrees (c 1.0, H2O); total neutral sugar content 96.60% w/w; weight-average molecular weight (M-w) similar to 360 kDa). Structural characterisation was performed by monosaccharide analysis and linkage analysis (methylation analysis, periodate oxidation and Smith degradation) on full and partial acid hydrolysates, followed by alditol acetylation, and product quantification by GC/GC-MS. Spectroscopic analysis (FT-IR and H-1/C-13 NMR) was also performed. Polysaccharide PS-1 was found to be an alpha-D-glucan with a (1 --> 4)-linked alpha-D-Glcp backbone, with (1 --> 6)-linked (1 --> 4)-alpha-D-Glcp side chains, side chains being short in length with no additional branching, and a minimum branching of 1 in every 9-11 backbone units. The distribution of side chains lengths and corresponding branching density requires further investigation. (C) 2010 Elsevier Ltd. All rights reserved.
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