Technology optimization for polysaccharides (POP) extraction from the fruiting bodies of Pleurotus ostreatus by Box-Behnken statistical design

Response Surface methodology (RSM), based on A Box-Behnken design (BBD), was used to optimize the extraction conditions of polysaccharides (POP) from the fruiting bodies of Pleurotus ostreatus. Four independent variables such as extraction temperature (degrees C), ratio of water to raw material, number of extraction and extraction time (h) were investigated. The experimental data obtained were fitted to a second-order polynomial equation Using Multiple regression analysis and also analyzed by appropriate statistical methods. The optimum extraction conditions, determined by the 3-D response surface and contour plots derived from the mathematical models, were as follows: extraction temperature 94.9 degrees C, number of extraction 4 ratio of water to raw material 22 and extraction time 2.7 h. Under these conditions, the experimental value was 64.1 +/- 1.42, which is well matched with value predicted by the model. (C) 2009 Elsevier Ltd. All rights reserved.