Journal
CARBOHYDRATE POLYMERS
Volume 82, Issue 3, Pages 578-584Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.05.011
Keywords
Galactose moiety; N-Isopropylacrylamide; Temperature-responsive; Hydrogel; Cell culture
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Funding
- National Nature Science Foundation of China [20574051]
- Science and Technology Commission Foundation of Tianjin [09JCZDJC23100]
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A novel thermo-responsive and biocompatible copolymer hydrogel based on N-isopropylacrylamide (NIPAAm) and galactosylated acrylate (GAC) was synthesized successfully by the free radical copolymerization. The copolymer hydrogel poly(NIPAAm-co-GAC) exhibits reversible temperature-response at 30 C in both water and cell culture medium. Compared with conventional PNIPAAm hydrogel, the adhesion and proliferation of HepG2 and L929 cells were higher on surfaces of hydrogels, suggesting that the incorporation of GAC could stimulate cell adsorption and growth. In addition, both HepG2 and L929 cells could spontaneously detach from the surface of the poly(NIPAAm-co-GAC) hydrogels without treating with any enzymes, and the detached cells at 20 degrees C showed higher viability than those detached from that on PNIPAAm hydrogel. (C) 2010 Elsevier Ltd. All rights reserved.
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