Journal
CARBOHYDRATE POLYMERS
Volume 78, Issue 2, Pages 275-281Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.03.046
Keywords
Paenibacillus polymyxa; Endophytic bacterium; Exopolysaccharides; Culture conditions; Antioxidant activity
Categories
Funding
- National Natural Science Foundation of China [30570415, 30870547]
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The production, characterization and antioxidant activities in vitro of exopolysaccharides (EPS) from endophytic bacterium Paenibacillus polymyxa EJS-3 were investigated. For EPS production, the preferable culture conditions were 24 degrees C and pH 8 for 60 h with sucrose and yeast extract as the carbon and nitrogen sources, respectively. Notably, sucrose concentration was the prominent factor, and the maximum yield of EPS (22.82 g/L) was obtained at a sucrose concentration of 160 g/L The crude EPS was purified by chromatography of DEAE-52 and Sephadex G-100, affording EPS-1 and EPS-2 with molecular weights of 1.22 x 10(6) and 8.69 x 10(5) Da. respectively. They were composed of mannose, fructose and glucose in a molar ratio of 2.59:29.83:1 and 4.23:36.59:1, respectively. In addition, both crude and purified EPS showed strong scavenging activities on superoxide and hydroxyl radicals, and their antioxidant activities decreased in the order of crude EPS > EPS-2 > EPS-1. (C) 2009 Elsevier Ltd. All rights reserved.
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