Journal
CARBOHYDRATE POLYMERS
Volume 78, Issue 1, Pages 89-94Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.03.040
Keywords
Aurtabasidium pullulans; Exopolysaccharide; Pullulan; Downstream processing; Pullulanase; Nuclear magnetic resonance
Categories
Ask authors/readers for more resources
Exopolysaccharide produced by a new novel colour variant strain of Aureobasidium pullulans FB-1 was purified by cell harvesting and precipitation of the polymer. Various organic solvents were screened for pullulan precipitation. Isolation and purification of pullulan from fermentation broth was carried out using single-step purification strategy by isopropyl alcohol precipitation. Ratio of culture supernatant to isopropyl alcohol and time of precipitation were optimized for pullulan precipitation. Maximum yield (4.47%, w/v) of polysaccharide was obtained when two volumes of ice-cold isopropyl alcohol were added to one volume of supernatant with precipitation time of 12 h. IR spectra as well as carbon-13 and proton NMR spectra in aqueous solution of intact polysaccharide obtained from A. pullulans FB-1 and commercially available pullulan (Sigma, USA) revealed solely alpha-(1 -> 6) linked maltosyl units, in accord with the generally accepted structure of pullulan. Maximum hydrolysis (94.25%) of purified pullulan at 50 degrees C by pullulanase was achieved under agitation (150 rpm) after 360 min. (C) 2009 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available