Journal
CARBOHYDRATE POLYMERS
Volume 75, Issue 3, Pages 521-527Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2008.08.021
Keywords
Kenaf xylan; Xylanase; Streptomyces; Aldopentaouronic acid; Aldoteraouronic acid; NMR spectrometry; Ion exchange chromatography
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Funding
- College of Bioresource Science, Nihon-University
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Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked beta-D-xylopyranose (Xylp), some of which carry alpha-(1-2)-linked 4-O-methylglucopyranosyl acid (MeGlcAp) and glucopyranosyluronic acid (GlcAp) as side chain was digested with the xylanase preparation. At the final stage of digestion, main neutral oligosaccharides were xylotriose (Xyl(3)) and xylobiose (Xyl(2)) followed by xylotetraose (Xyl(4)). Xylose was only formed in a trace amount. As acidic oligosaccharides liberated, following seven aldopentao- and aldotetraouronic acids were isolated in a pure state, respectively, by preparative anion-exchange chromatography. Their structures were determined by analysis of constitutional sugar residues and H-1 and C-13 NMR spectroscopy. Xyl-beta-1,4-[MeGlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-Xyl; [MeGlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-Xyl-beta-1,4-Xyl; Xyl-beta-1,4-[GlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-Xyl [GlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-Xyl-beta-1,4-Xyl [MeGlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-D-Xyl [GlcA-alpha-1,2-]Xyl-beta-1,4-Xyl-beta-1,4-Xyl Xyl-beta-1,4-Xyl-beta-1,3-Rha-alpha-1,2-GalA-alpha-1,4-Xyl (C) 2008 Elsevier Ltd. All rights reserved.
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