Journal
CARBOHYDRATE POLYMERS
Volume 77, Issue 2, Pages 394-401Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.01.018
Keywords
Chitosan; beta-Cyclodextrin; Nanocomplex; Drug delivery; Insulin
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Funding
- Ministry of Education
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The objective of this work was to investigate the possibility of chitosan bearing beta-cyclodextrin (CDen-g-CS) nanocomplexes for controlled protein release. CDen-g-CS was synthesized by a one-step procedure with N-succinylated chitosan and mono(6-(2-aminoethyl)amino-6-deoxy)-beta-cyclodextrin in the presence of the water-soluble carbodiimide. The amount of beta-CD grafted was up to 62.1 wt%. In vitro cytotoxicity against NIH 3T3 cells showed that CDen-g-CS was not cytotoxic and no significant difference of cytotoxicity was found between CDen-g-CS groups. Self-assembled nanocomplexes between CDen-g-CS and insulin were in the size range of 190-328 nm, with positive electrical charge (+3.7 to +25.5 mV) and high loading efficiency (37.7%). Insulin release in vitro was affected by the medium pH and the composition of copolymer. These results demonstrated that CDen-g-CS copolymer was a new promising vehicle for controlled protein release. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
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