Journal
CARBOHYDRATE POLYMERS
Volume 71, Issue 3, Pages 411-415Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2007.06.011
Keywords
maize straw; polysaccharides; enzymatic hydrolysis; cellulase; cellobiase
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Enzymatic hydrolysis of maize straw polysaccharides was investigated for the production of reducing sugars. After maize straw was pretreated with 2% sodium hydroxide at 80 degrees C for 1 h to delignify, the cellulosic residues were hydrolyzed by cellulase from Trichoderma reesei ZU-02 and the hydrolysis yield at 48 h was 65.9%. A certain amount of cellobiose was accumulated in the hydrolysate due to low cellobiase activity in T reesei cellulase. Supplementing cellobiase from Aspergillus niger ZU-07 greatly reduced the inhibitory effect caused by cellobiose, and the hydrolysis yield at 48 h was improved to 81.2% with cellobiase activity enhanced to 10 CBU/g Substrate. The addition of 5 g/l Tween-80 improved the enzymatic hydrolysis by increasing the hydrolysis yield of 7.5%. Fed-batch hydrolysis was started with a batch hydrolysis containing 80 g/l substrate, with cellulosic residues added at 6 and 12 It to get a final substrate concentration of 110 g/l. After 72 h of hydrolysis, the reducing sugars concentration reached 89.5 g/l with a hydrolysis yield of 83.3%. The hydrolysate from fed-batch hydrolysis contained 56.7 g/l glucose, 23.6 g/l xylose, and 5.7 g/l arabinose, which is suitable for subsequent fermentation process. (C) 2007 Elsevier Ltd. All rights reserved.
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