Journal
CARBOHYDRATE POLYMERS
Volume 74, Issue 3, Pages 504-508Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2008.04.019
Keywords
Serratia marcescens FS-3; crab shell; chitin extraction; Delvolase (R)
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Funding
- Korea Science and Engineering Foundation (KOSEF)
- Ministry of Science and Technology [R0A-2003-000-10322-0(2007)]
- National Research Foundation of Korea [핵06B2515, R0A-2003-000-10322-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Serratia marcescens FS-3, isolated from a soil sample in the southwestern area of Korea, exhibited a strong protease activity of 60.7 U/ml after 3 days of incubation. The protein removal from natural crab shell wastes with 10% S. marcescens FS-3 inoculum was 84% after 7 days of fermentation. At the same time, 47% demineralization occurred. When the shell waste was treated with 1% Delvolase(R) as a reference, deproteinization rate was 90%. In combination of 10% S. marcescens FS-3 culture supernatant and 1% Delvolase(R), deproteinization rate of the shell waste was 85%, while the rate was 81% in 10% FS-3 Culture Supernatant only. These results suggest that bio-deproteinization of the crab shell wastes using S. marcescens FS-3 Could be applicable to the chitin production process. (C) 2008 Elsevier Ltd. All rights reserved.
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